The Department of Cryopreservation
Started functioning in 2004, while primarily focused on IVF patients to store the surplus embryos and semen of husbands traveling abroad. The current issues related to female and male infertility depicts the impact of environmental and physical changes due to various reasons such as obesity, change in lifestyle and diet, use of chemical substances as preservatives, radiation, use of pesticides and insecticides in agriculture, dumping and burning of garbage leading to air, soil and water pollution, etc. Due to these impacts on the physical body, males and females are experiencing various health ailments like cancer, liver cirrhosis, diabetes, infectious diseases, etc., which leads to infertility due to the usage of chemotherapies or radiotherapies for treatment. Also due to smoking, the use of alcohol, narcotic drugs, emotional stress, etc. causes effects of low sperm count in males as well as abnormal oocyte in the female.
As cancer treatments improve, the problems faced by survivors of cancer and the complications of cancer therapies become more important. Many survivors are young and are diagnosed and treated before they have children. Cancer and its treatment can significantly affect the chance of a patient having a child in the future.
Prasad Infertility solutions, the Top Infertility Hospitals in Hyderabad, therefore realize how important it is that all patients are able to discuss the effects of treatment with a fertility expert and explore whether they are able to store eggs, sperm or embryos before their cancer treatment, which could be used to help them have their biological child in the future.
Cancer and Infertility are two devastating diagnoses simultaneously affecting young men and women. Having to face both diagnoses can cause huge distress and therefore all patients will be offered an appointment to see one of our counselors if they wish to discuss fertility preservation.
Cryopreservation is a method in which the male (sperm) and female (oocyte) gametes can be preserved at the low temperature of -1960C in Liquid Nitrogen for long periods and can be retrieved when it is needed for pregnancy planning.
- • Prasad Infertility solutions have stored sperm for men and oocyte as well as embryos for couples going through infertility treatments and those facing cancer treatments.
- • Our Fertility Preservation service for sperms, oocytes, and embryos started in 2004.
- • We suggest the patients planning cancer therapies to approach at least a week before the actual cancer treatment.
- • We offer patient-centric Cryo freezing methods to eligible patients. We continue to work with Cancer specialists to ensure that after completing treatment, the patient can retrieve the frozen sample for pregnancy planning.
Cryopreservation of Testicular Tissue
- • Testicular tissue cryopreservation is an option for those who can’t produce sperm and are unable to preserve the semen sample.
- • Many numbers of patients have cryopreserved their testicular tissue.
- • Spermatogenic recovery or pregnancies from cryopreserved testicular tissues have now been reported with successful results.
- • Many methods are suggested that preserve testicular cells for many years to restore fertility.
- • The young patients who have the risk of infertility can preserve testicular tissue.
Ovarian Cortical Strips
- Preservation can be done for those who have issues like delaying marriage, suffering from hematologic malignancies or breast cancer, who have the risk of ovarian failure. This procedure is as feasible and safe as comparable operative procedures in children.
Procedures Involved in Cryopreservation of Ovarian Tissue
- • A part of the ovary is taken and should get slowly frozen before storing it in liquid nitrogen.
- • The tissues can then be thawed where the cells start producing new eggs. This allows normal conception to take place with the eggs retrieved.
Oocyte (egg) Freezing
- • Women may attempt to ‘freeze’ eggs/oocyte for future use before they undergo treatment such as chemotherapy, which may affect their eggs.
- • Oocytes (eggs) are stimulated to develop within the ovaries by daily administration of hormone injections. Following this, the eggs are removed from the ovaries under ultrasound control.
- • The eggs removed are then frozen very rapidly using the technique of Vitrification.
- • Eggs can be stored for up to 10 years.
- • Vitrification is the most effective way of freezing human eggs with more than 90% of vitrified eggs surviving after thawing.
- • The number of pregnancies depends on the number of eggs stored, with each egg having approximately a 5% chance of leading to pregnancy in women under 35 years.
- • Sperm cryopreservation or sperm freezing is a method for men to preserve their sperm and store it in a bank so it can be used in the future.
- • Men who are frequent travelers can utilize this wonderful technology to store their sperm and retrieved it when the wife is ovulating.
- • For couples who travel to our center for fertility treatment, sperm cryopreservation is a practical way to plan their travel.
Procedure for Sperm Freezing
- • The male partner based on the reference of Fertility expert will be advised to maintain an absence of sexual intercourse or masturbation for a period of 3 to 5 days before sperm freezing.
- • The semen sample is collected by the masturbation method in a sterile container and kept in a 37oC warm water bath for liquefaction of semen.
- • The liquefied semen is mixed in 1:1 ration in freezing medium and this allows your sperm to survive the freezing and storage.
- • The Semen samples are frozen in liquid nitrogen vapors. After frozen, they are stored in liquid nitrogen until needed.
- • Each ejaculate can yield between one to three vials.
- • Each vial of the semen sample is labeled with the patient’s name, the date, and a unique number.
Test Thaw Procedure
- • After the cryopreservation, the survival rate of sperm may vary.
- • Depending on the amount of sperm you preserved, the cryovials are examined and thawed.
Embryo Cryopreservation (Freezing)
- • If a woman is in a stable relationship, the couple may wish to freeze embryos instead of eggs.
- • The woman undergoes a cycle of ovarian stimulation and egg retrieval as in conventional IVF. On the day of egg collection, the male partner is asked to provide a fresh sample of semen, produced on-site in the Andrology Department.
- • All mature eggs retrieved are injected with a single sperm using a technique known as ICSI (Intra Cytoplasmic Sperm Injection). On average, about 50-60% fertilizes and form embryos.
- • The laboratory will ring you the morning after egg collection to tell you how many eggs fertilized.
- • The embryos at the 6 to 8 cell stage will be vitrified for fertility preservation.
- • Embryo freezing is a relatively successful procedure and follow-up studies on babies born are reassuring.
- • If the Woman is under 35 years, approximately 1 out of 3 couples will conceive following embryo freezing.
- • Embryo storage should only be carried out for couples in a stable relationship as, if the couple separates, the male partner may withdraw his consent for continued storage and treatment. As a result, the embryos would have to perish.
Cryopreservation Experience at Prasad Infertility Solutions
The 1st successful frozen embryo transfer was performed in 2005, which was a blastocyst (day 5 embryo). Interestingly, it was a single embryo from the couple, frozen for specific reasons. The woman came back after 3 months and had the single frozen-thawed embryo transfer which resulted in a healthy male baby. The family, so exhilarated to date, celebrate every birthday of the child with Dr. Suma Prasad.